It's very helpful if you add a bulk delete option in a Selected Assortment Under Promotion Tab, instead Single Row Delete option. Please Find the Attached picture for more.
We already have an import excel functionality for this which you can use for deleting all the barcodes at once. Use Import class: 'Promotion Assortment - Item Addition' for the same. You will find a provision where system will ask you behaviour, i.e., Specify whether to clear the existing assortment details and then populate the new ones or just to append to the existing ones.
Please give more information: Why do you need to delete all items from an existing assortment? Why aren't you creating a new assortment instead and mark existing one extinct?
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